A plasmid which does not encode the aminoglycoside phosphotransferase in the butirosin-producing strain of Bacillus circulans.
نویسندگان
چکیده
Bacillus circulans NRRL B-3312 produces the aminoglycoside antibiotic butirosin and encodes an aminoglycoside 3'-phosphotransferase. We detected a 48 kilobase plasmid, pIP850, in this strain; this was analyzed by agarose gel electrophoresis following digestion with EcoRI restriction endonuclease and by nucleic acid hybridization. The results obtained indicate that plasmid pIP850 does not carry the structural gene for the aminoglycoside modifying enzyme.
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ورودعنوان ژورنال:
- The Journal of antibiotics
دوره 35 5 شماره
صفحات -
تاریخ انتشار 1982